Key features and details
- Rabbit polyclonal to Zonula occludens protein 3/ZO3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Zonula occludens protein 3/ZO3 antibody
See all Zonula occludens protein 3/ZO3 primary antibodies
DescriptionRabbit polyclonal to Zonula occludens protein 3/ZO3
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- Human colon tissue, CACO-2 cells
This product was previously labelled as Zonula occludens protein 3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Cellular localizationCell membrane. Cell junction > tight junction.
- Information by UniProt
- Tight junction protein 3 antibody
- Tight junction protein ZO-3 antibody
- Tight junction protein ZO3 antibody
Immunofluorescence analysis of CACO-2 cells labeling Zonula occludens protein 3/ZO3 with ab204231 at 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon tissue labeling Zonula occludens protein 3/ZO3 with ab204231 at 1/200 dilution.
ab204231 has not yet been referenced specifically in any publications.