Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (ab191143)
Key features and details
- Rabbit polyclonal to Zonula occludens protein 3/ZO3 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal
See all Zonula occludens protein 3/ZO3 primary antibodies -
Description
Rabbit polyclonal to Zonula occludens protein 3/ZO3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Dog -
Immunogen
Synthetic peptide corresponding to Mouse Zonula occludens protein 3/ZO3 aa 850 to the C-terminus (C terminal).
Database link: Q9QXY1 -
Positive control
- Rat intestine and Mouse liver tissues; Rat brain and heart tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 99 kDa.
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Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 99 kDa. |
Target
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Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
Cellular localization
Cell membrane. Cell junction > tight junction. - Information by UniProt
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Database links
- Entrez Gene: 27375 Mouse
- Entrez Gene: 314640 Rat
- SwissProt: O62683 Dog
- SwissProt: Q9QXY1 Mouse
- Unigene: 27984 Mouse
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Alternative names
- Tight junction protein 3 antibody
- Tight junction protein ZO-3 antibody
- Tight junction protein ZO3 antibody
see all
Images
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All lanes : Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (ab191143) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat heart tissue lysate
Predicted band size: 99 kDa -
Immunohistochemical analysis of Paraffin-embedded Mouse liver tissue labeling Zonula occludens protein 3/ZO3 with ab191143 at 1 µg/ml.
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Immunohistochemical analysis of Paraffin-embedded Rat intestine tissue labeling Zonula occludens protein 3/ZO3 with ab191143 at 1 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab191143 has been referenced in 4 publications.
- Corsetti G et al. Qualitative Nitrogen Malnutrition Damages Gut and Alters Microbiome in Adult Mice. A Preliminary Histopathological Study. Nutrients 13:N/A (2021). PubMed: 33810512
- Wang L et al. Methylation of the miR-29b-3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer. Oncol Rep 45:65-72 (2021). PubMed: 33416135
- Lee YC et al. Acorus tatarinowii Schott extract reduces cerebral edema caused by ischemia-reperfusion injury in rats: involvement in regulation of astrocytic NKCC1/AQP4 and JNK/iNOS-mediated signaling. BMC Complement Med Ther 20:374 (2020). PubMed: 33298024
- Shaalan A et al. Epithelial disruptions, but not immune cell invasion, induced secretory dysfunction following innate immune activation in a novel model of acute salivary gland injury. J Oral Pathol Med 47:211-219 (2018). PubMed: 29160910