Overview

  • Product name

    Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal
    See all Zonula occludens protein 3/ZO3 primary antibodies
  • Description

    Rabbit polyclonal to Zonula occludens protein 3/ZO3 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide corresponding to Mouse Zonula occludens protein 3/ZO3 aa 885-905 (C terminal).
    Sequence:

    RARDVESSDDEGYDWGPATDL


    Database link: Q9QXY1

  • Positive control

    • Rat intestine and Mouse liver tissues; Rat brain and heart tissue lysates.
  • General notes

     This product was previously labelled as Zonula occludens protein 3

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 99 kDa.

Target

  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell membrane. Cell junction > tight junction.
  • Information by UniProt
  • Database links

  • Alternative names

    • Tight junction protein 3 antibody
    • Tight junction protein ZO-3 antibody
    • Tight junction protein ZO3 antibody
    • TJP3 antibody
    • ZO3 antibody
    • ZO3_HUMAN antibody
    • Zona occludens protein 3 antibody
    • Zonula occludens protein 3 antibody
    see all

Images

  • All lanes : Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (ab191143) at 0.5 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat heart tissue lysate

    Predicted band size: 99 kDa

  • Immunohistochemical analysis of Paraffin-embedded Mouse liver tissue labeling Zonula occludens protein 3/ZO3 with ab191143 at 1 µg/ml.

  • Immunohistochemical analysis of Paraffin-embedded Rat intestine tissue labeling Zonula occludens protein 3/ZO3 with ab191143 at 1 µg/ml.

References

ab191143 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191143.
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