Recombinant
RabMAb

Recombinant Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] (ab182159)

Overview

  • Product name

    Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152]
    See all Zonula occludens protein 3/ZO3 primary antibodies
  • Description

    Rabbit monoclonal [EPR13414-152] to Zonula occludens protein 3/ZO3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    within Human Zonula occludens protein 3/ZO3 aa 800 to the C-terminus. The exact sequence is proprietary.
    Database link: O95049

  • Positive control

    • Caco-2, MCF-7 cell lysates. Human fetal kidney tissue lysate.
  • General notes

     

     

     This product was previously labelled as Zonula occludens protein 3

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13414-152
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 103 kDa).

Target

  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell membrane. Cell junction > tight junction.
  • Information by UniProt
  • Database links

  • Alternative names

    • Tight junction protein 3 antibody
    • Tight junction protein ZO-3 antibody
    • Tight junction protein ZO3 antibody
    • TJP3 antibody
    • ZO3 antibody
    • ZO3_HUMAN antibody
    • Zona occludens protein 3 antibody
    • Zonula occludens protein 3 antibody
    see all

Images

  • All lanes : Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] (ab182159) at 1/50000 dilution

    Lane 1 : Caco-2 cell lysate
    Lane 2 : MCF-7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?

  • Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] (ab182159) at 1/10000 dilution + Human fetal kidney tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?

References

ab182159 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate 0.05% Tween-20
Permeabilization
No
Specification
Testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 29 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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