Recombinant Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free (ab250601)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13414-152] to Zonula occludens protein 3/ZO3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free
See all Zonula occludens protein 3/ZO3 primary antibodies -
Description
Rabbit monoclonal [EPR13414-152] to Zonula occludens protein 3/ZO3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250601 is the carrier-free version of ab182159 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab250601 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Zonula occludens protein 3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13414-152 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 103 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 103 kDa). |
Target
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Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
Cellular localization
Cell membrane. Cell junction > tight junction. - Information by UniProt
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Database links
- Entrez Gene: 27134 Human
- Entrez Gene: 314640 Rat
- Omim: 612689 Human
- SwissProt: O95049 Human
- Unigene: 25527 Human
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Alternative names
- Tight junction protein 3 antibody
- Tight junction protein ZO-3 antibody
- Tight junction protein ZO3 antibody
see all
Images
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Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free (ab250601)All lanes : Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] (ab182159) at 1/50000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : MCF-7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?This data was developed using ab182159, the same antibody clone in a different buffer formulation.
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Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free (ab250601)Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] (ab182159) at 1/10000 dilution + Human fetal kidney tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?This data was developed using ab182159, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab250601 has not yet been referenced specifically in any publications.