Key features and details
- Rabbit polyclonal to ZP2
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-ZP2 antibody
See all ZP2 primary antibodies
DescriptionRabbit polyclonal to ZP2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat
Recombinant fragment (His-T7-tag) corresponding to Rat ZP2 aa 353-616. (Expressed in E.coli)
CTRDGFMDFEVYSHQTKPALNLESLLVGNSSCQPIFKVQSLGLARFHIPL NGCGTRQKFEGDKVIYENEIHALWENPPSNIIFRNSEFRMTVRCHYIRDS MLLRAHIKSHSSPVASVKPGPLALVLQTYPDISYQRPYRKNEYPLVRYLR QPIYMEVTVLNRNDPNIKLVLDDCWATTFEDPASVPQWQIIMDGCEYELD NYRTTFHAANSSAAHSGHYQRFDVKTFAFVSESRGLSSLIYFHCSALICN QASPLCSVTCPAP
Database link: O54767
- WB: Rat ovary and placenta tissue; Recombinant rat ZP2 protein. IHC-P: Rat ovary tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 78 kDa.|
FunctionThe mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
Sequence similaritiesBelongs to the ZP domain family. ZPA subfamily.
Contains 1 ZP domain.
Developmental stageExpressed during the 2-week growth phase of oogenesis, prior to ovulation.
DomainThe ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
modificationsProteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
Proteolytically cleaved in the N-terminal part after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy.
O-glycosylated; contains sulfate-substituted glycans.
Cellular localizationSecreted > extracellular space > extracellular matrix and Cell membrane.
- Information by UniProt
- OTTHUMP00000115849 antibody
- Processed zona pellucida sperm-binding protein 2 antibody
- Zona pellucida glycoprotein 2 antibody
All lanes : Anti-ZP2 antibody (ab231354) at 1 µg/ml
Lane 1 : Rat ovary tissue lysate.
Lane 2 : Rat placenta tissue lysate.
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 78 kDa
Anti-ZP2 antibody (ab231354) at 1 µg/ml + Recombinant rat ZP2 protein.
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 78 kDa
Formalin-fixed, paraffin-embedded rat ovary tissue stained for ZP2 using ab231354 at 20 µg/mL in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231354 has not yet been referenced specifically in any publications.