Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab48895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.6 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionThe mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
Sequence similaritiesBelongs to the ZP domain family. ZPC subfamily.
Contains 1 ZP domain.
DomainThe ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
modificationsProteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.
Cellular localizationSecreted > extracellular space > extracellular matrix and Cell membrane.
- Information by UniProt
- HUMZP3 antibody
- Processed zona pellucida sperm-binding protein 3 antibody
- Sperm receptor antibody
Anti-ZP3 antibody (ab48895) at 0.6 µg/ml + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Diluted in 5% skim milk / PBS buffer, gel concentration 12%
ab48895 has not yet been referenced specifically in any publications.