Anti-ZP3 antibody (ab70384)
Key features and details
- Rabbit polyclonal to ZP3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZP3 antibody -
Description
Rabbit polyclonal to ZP3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ZP3 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab87447) -
Positive control
- This antibody gave a positive signal in JEG-3 Whole Cell Lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation. -
Tissue specificity
Oocytes. -
Sequence similarities
Belongs to the ZP domain family. ZPC subfamily.
Contains 1 ZP domain. -
Domain
The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida. -
Post-translational
modificationsProteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
N-glycosylated.
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy. -
Cellular localization
Secreted > extracellular space > extracellular matrix and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7784 Human
- Omim: 182889 Human
- SwissProt: P21754 Human
- Unigene: 656137 Human
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Alternative names
- HUMZP3 antibody
- Processed zona pellucida sperm-binding protein 3 antibody
- Sperm receptor antibody
see all
Images
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Anti-ZP3 antibody (ab70384) at 1 µg/ml + JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 17 kDa (possible cleavage fragment)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab70384 has been referenced in 1 publication.
- Du D et al. Senotherapy Protects against Cisplatin-Induced Ovarian Injury by Removing Senescent Cells and Alleviating DNA Damage. Oxid Med Cell Longev 2022:9144644 (2022). PubMed: 35693700