Overview

  • Product name

  • Description

    Rabbit polyclonal to ZPBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 29-285 of Human ZPBP1 (NP_008940).

  • Positive control

    • WB: 293T, A431, H1299 or HeLaS3 cell lysate; IHC-P: SCC25 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab97691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P 1/100.

Target

  • Relevance

    ZPBP1 is one of several proteins that are thought to participate in secondary binding between acrosome-reacted sperm and the egg-specific extracellular matrix, the zona pellucida
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • 4930486K01Rik antibody
    • FLJ9322 antibody
    • Iam38 antibody
    • OTTHUMP00000208840 antibody
    • OTTMUSP00000008129 antibody
    • RP23-446K7.1 antibody
    • Sp38 antibody
    • Zona pellucida binding protein antibody
    • Zona pellucida binding protein 1 antibody
    • ZPBP antibody
    see all

Images

  • All lanes : Anti-ZPBP1 antibody (ab97691) at 1/500 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 40 kDa



    12% SDS-PAGE.
  • ab97691 at 1/100 dilution staining ZPBP1 in paraffin-embedded SCC25 xenograft tissue by immunohistochemistry.

References

This product has been referenced in:

  • Liu X  et al. iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failure. Clin Proteomics 15:27 (2018). Read more (PubMed: 30166971) »
See 1 Publication for this product

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