Product nameAnti-ZPR9 antibody
See all ZPR9 primary antibodies
DescriptionRabbit polyclonal to ZPR9
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Giant Panda
Synthetic peptide within Human ZPR9 aa 25-75. The exact sequence is proprietary. (NP_219482.1).
Database link: Q969S3
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
This product was previously labelled as ZNF622
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226174 was affinity purified using an epitope specific to ZPR9 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 54 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay behave as an activator of the bound transcription factor, MYBL2, and be involved in embryonic development.
Tissue specificityExpressed in lung, kidney, spleen, liver and brain with lowest expression in kidney.
Sequence similaritiesContains 2 U1-type zinc fingers.
modificationsPhosphorylated by MELK. The phosphorylation may redirect the protein to the nucleus.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- MGC108936 antibody
- Zfp622 antibody
- Zinc finger protein 622 antibody
All lanes : Anti-ZPR9 antibody (ab226174) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 10 seconds
ZPR9 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226174 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226174 at 0.4 µg/ml.
Lane 1: ab226174 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab226174 has not yet been referenced specifically in any publications.