Anti-ZRANB1 antibody (ab82132)
Key features and details
- Goat polyclonal to ZRANB1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZRANB1 antibody
See all ZRANB1 primary antibodies -
Description
Goat polyclonal to ZRANB1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Synthetic peptide:
KWLDRYRQIRPCTS
, corresponding to C terminal amino acids 682-695 of Human ZRANB1 -
Positive control
- Human Cerebellum Lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 81 kDa.
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 81 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Positive regulator of the Wnt signaling pathway that specifically cleaves 'Lys-63'-linked ubiquitin chains. Acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription. May also modulate TNF-alpha signaling. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the peptidase C64 family.
Contains 1 OTU domain.
Contains 3 RanBP2-type zinc fingers. -
Domain
The OTU domain mediates the deubiquitinating activity.
The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 54764 Human
- Entrez Gene: 360216 Mouse
- Omim: 611749 Human
- SwissProt: Q9UGI0 Human
- SwissProt: Q7M760 Mouse
- Unigene: 595158 Human
- Unigene: 389984 Mouse
- Unigene: 397069 Mouse
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Alternative names
- DKFZp762P2216 antibody
- hTrabid antibody
- Trabid antibody
see all
Images
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Western blot - Anti-ZRANB1 antibody (ab82132)Anti-ZRANB1 antibody (ab82132) at 0.1 µg/ml + Human Cerebellum Lysate (35ug in RIPA buffer)
Predicted band size: 81 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab82132 has not yet been referenced specifically in any publications.