- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-ZRANB1 antibody
See all ZRANB1 primary antibodies -
DescriptionGoat polyclonal to ZRANB1
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Host speciesGoat
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Synthetic peptide:
KWLDRYRQIRPCTS
, corresponding to C terminal amino acids 682-695 of Human ZRANB1 -
Positive control
- Human Cerebellum Lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab82132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 81 kDa. | |
| ELISA | Use at an assay dependent dilution. |
Target
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FunctionPositive regulator of the Wnt signaling pathway that specifically cleaves 'Lys-63'-linked ubiquitin chains. Acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription. May also modulate TNF-alpha signaling.
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Tissue specificityWidely expressed.
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Sequence similaritiesBelongs to the peptidase C64 family.
Contains 1 OTU domain.
Contains 3 RanBP2-type zinc fingers. -
DomainThe OTU domain mediates the deubiquitinating activity.
The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 54764 Human
- Entrez Gene: 360216 Mouse
- Omim: 611749 Human
- SwissProt: Q9UGI0 Human
- SwissProt: Q7M760 Mouse
- Unigene: 595158 Human
- Unigene: 389984 Mouse
- Unigene: 397069 Mouse
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Alternative names
- DKFZp762P2216 antibody
- hTrabid antibody
- Trabid antibody
see all
Images
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Western blot - Anti-ZRANB1 antibody (ab82132)Anti-ZRANB1 antibody (ab82132) at 0.1 µg/ml + Human Cerebellum Lysate (35ug in RIPA buffer)
Predicted band size: 81 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Protocols
References
ab82132 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"