Anti-ZRSR2 antibody (ab223062)
Key features and details
- Rabbit polyclonal to ZRSR2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ZRSR2 antibody -
Description
Rabbit polyclonal to ZRSR2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human ZRSR2 aa 150-400.
Database link: Q15696 -
Positive control
- WB: K562 and HEK-293 wole cell lysates; mouse heart tissue lysate. IHC-P: Human testis tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50 - 1/200.
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WB |
1/500 - 1/5000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/20 - 1/200.
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Notes |
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ICC/IF
1/50 - 1/200. |
WB
1/500 - 1/5000. Predicted molecular weight: 58 kDa. |
IHC-P
1/20 - 1/200. |
Target
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Function
Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 2 C3H1-type zinc fingers.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated in the RS domain by SRPK1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8233 Human
- Entrez Gene: 22184 Mouse
- Omim: 300028 Human
- SwissProt: Q15696 Human
- SwissProt: Q62377 Mouse
- Unigene: 171909 Human
- Unigene: 426178 Mouse
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Alternative names
- CCCH type zinc finger antibody
- CCCH type zinc finger, RNA binding motif and serine/arginine rich protein 2 antibody
- MGC142014 antibody
see all
Images
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All lanes : Anti-ZRSR2 antibody (ab223062) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : mouse heart tissue lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa -
Paraffin embedded human testis tissue stained for ZRSR2 with ab223062 (1/100) in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ZRSR2 (green) using ab223062 (1/100) in ICC/IF. Secondary: Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223062 has not yet been referenced specifically in any publications.