Key features and details
- Rabbit polyclonal to ZRSR2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ZRSR2 antibody
DescriptionRabbit polyclonal to ZRSR2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human ZRSR2 aa 191-372.
HNFPTSSPTLLIKSMFTTFGMEQCRRDDYDPDASLEYSEEETYQQFLDFY EDVLPEFKNVGKVIQFKVSCNLEPHLRGNVYVQYQSEEECQAALSLFNGR WYAGRQLQCEFCPVTRWKMAICGLFEIQQCPRGKHCNFLHVFRNPNNEFW EANRDIYLSPDRTGSSFGKNSERRERMGHHDD
Database link: Q15696
- WB: K562 and HEK-293 wole cell lysates; mouse heart tissue lysate. IHC-P: Human testis tissue. ICC/IF: HepG2 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab223062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 58 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionPre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing.
Tissue specificityWidely expressed.
Sequence similaritiesContains 2 C3H1-type zinc fingers.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated in the RS domain by SRPK1.
- Information by UniProt
- CCCH type zinc finger antibody
- CCCH type zinc finger, RNA binding motif and serine/arginine rich protein 2 antibody
- MGC142014 antibody
All lanes : Anti-ZRSR2 antibody (ab223062) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : mouse heart tissue lysate
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa
Paraffin embedded human testis tissue stained for ZRSR2 with ab223062 (1/100) in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ZRSR2 (green) using ab223062 (1/100) in ICC/IF. Secondary: Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
ab223062 has not yet been referenced specifically in any publications.