Overview

  • Product name

  • Description

    Rabbit polyclonal to ZRSR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (KEQWEEQQRK EREEEEQKRQ EKKEKEEALQ KMLDQAENEL ENGTTWQNPE) of Human ZRSR2 (NP_005080).

  • Positive control

    • Human fetal spleen lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 2 C3H1-type zinc fingers.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated in the RS domain by SRPK1.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCCH type zinc finger antibody
    • CCCH type zinc finger, RNA binding motif and serine/arginine rich protein 2 antibody
    • MGC142014 antibody
    • MGC142040 antibody
    • Renal carcinoma antigen NY REN 20 antibody
    • Renal carcinoma antigen NY-REN-20 antibody
    • RNA-binding motif and serine/arginine rich protein 2 antibody
    • U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit related protein 2 antibody
    • U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2 antibody
    • U2(RNU2) small nuclear RNA auxiliary factor 1 like 2 antibody
    • U2(RNU2) small nuclear RNA auxiliary factor 1-like 2 antibody
    • U2AF1 RS2 antibody
    • U2AF1L2 antibody
    • U2AF1RS2 antibody
    • U2AF35-related protein antibody
    • U2AFM_HUMAN antibody
    • URP antibody
    • Zinc finger-, CCCH domain-, and RNA-binding motif-containing serine/arginine-rich protein 2 antibody
    • Zrsr2 antibody
    see all

Images

  • Anti-ZRSR2 antibody (ab94591) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal spleen lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 58 kDa



    Gel concentration: 12%

References

ab94591 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you very much for your interest in our antibodies.

I can confirm that both antibodies are not tested and guaranteed for mouse samples.

We usually expect an cross-reaction of an antibody when the immunogne shows 85% or more homology to a other species

The immunogen from ab50753 only shows a 71% homology and we therefore would not recommend thia antibody for mouse samples.

But ab94591 shows a 88% homology to mouse (Q62377).

Therefore, I can offer a discount off a future purchase if you buy ab94591 now, test it inmouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab94591 in mouse. I will then send a discount code. This code must be issued before purchasingab94591 so please wait for my reply before ordering.

2. Purchase ab94591 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab94591 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your email.

The immunogen of ab50753 also show similarity with mouse >90% so this antibody would also react with mouse samples.

I hope this bit of information will also be helpful.

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Answer

Thank you for contacting us.

The cross reactivity info we have for these products is entirely based on the sequence homology between human and mouse. The immunogen of ab94951 is about 88% (Blast homology) similar to the mouse sequence so this antibody is more likely to cross react. We however have no tested data to share. If you are interested in using this antibody, let me know I will be happy to provide a Abreview discount code. The discount code will the worth the price for one primary antibody. The terms and conditions can be checked by clicking the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I have inquired about the ab50753 cross reactivity to lab and now waiting a reply. I will send you the confirmation soon.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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