Overview

  • Product name

  • Description

    Rabbit polyclonal to ZSCAN4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    An 18 amino acid peptide from near the carboxy terminus of Human ZSCAN4 (NP_689890).

  • Positive control

    • Mouse lung tissue lysate; Rat lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab106646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa.

Target

  • Function

    Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.
  • Sequence similarities

    Contains 4 C2H2-type zinc fingers.
    Contains 1 SCAN box domain.
  • Cellular localization

    Nucleus. Chromosome > telomere.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ35105 antibody
    • MGC126787 antibody
    • MGC126789 antibody
    • Zinc finger and SCAN domain containing 4 antibody
    • Zinc finger and SCAN domain containing protein 4 antibody
    • Zinc finger and SCAN domain-containing protein 4 antibody
    • Zinc finger protein 494 antibody
    • ZNF 494 antibody
    • ZNF494 antibody
    • ZSCA4_HUMAN antibody
    • ZSCAN4 antibody
    see all

Images

  • Immunohistochemistry analysis of ZSCAN4 expression in fromalin-fixed, paraffin-embedded rat lung tissue with ab106646 at 5 µg/ml.
  • All lanes : Anti-ZSCAN4 antibody (ab106646) at 1 µg/ml

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse kidney tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 49 kDa

  • Immunofluorescence of ZSCAN4 in Rat Lung cells using ab106646 at 20 ug/ml.

References

ab106646 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab97748.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (SHEF3 hESC)
Loading amount
20 µg
Specification
SHEF3 hESC
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Mar 08 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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