Product nameAnti-ZSCAN4 antibody
See all ZSCAN4 primary antibodies
DescriptionRabbit polyclonal to ZSCAN4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla, Orangutan
- This antibody gave a positive signal in Human kidney tissue lysate as well as the following whole cell lysates: HEK293; A498; HuES7.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97748 in the following tested applications.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).|
FunctionEmbryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.
Sequence similaritiesContains 4 C2H2-type zinc fingers.
Contains 1 SCAN box domain.
Cellular localizationNucleus. Chromosome > telomere.
- Information by UniProt
- FLJ35105 antibody
- MGC126787 antibody
- MGC126789 antibody
All lanes : Anti-ZSCAN4 antibody (ab97748) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 4 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The predicted molecular weight of ZSCAN4 is 49 kDa (SwissProt), however we expect to observe a banding pattern at 58 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab97748 has not yet been referenced specifically in any publications.