Key features and details
- Rabbit polyclonal to ZSCAN4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZSCAN4 antibody
See all ZSCAN4 primary antibodies
DescriptionRabbit polyclonal to ZSCAN4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla, Orangutan
- This antibody gave a positive signal in Human kidney tissue lysate as well as the following whole cell lysates: HEK293; A498; HuES7.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).|
FunctionEmbryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.
Sequence similaritiesContains 4 C2H2-type zinc fingers.
Contains 1 SCAN box domain.
Cellular localizationNucleus. Chromosome > telomere.
- Information by UniProt
- FLJ35105 antibody
- MGC126787 antibody
- MGC126789 antibody
All lanes : Anti-ZSCAN4 antibody (ab97748) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 4 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The predicted molecular weight of ZSCAN4 is 49 kDa (SwissProt), however we expect to observe a banding pattern at 58 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab97748 has not yet been referenced specifically in any publications.