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Mouse monoclonal [3363C4a] to ZW10
ZW10 recombinant fragment, internal sequence, (Human).
The antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.05% Sodium azide
Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride
Concentration information loading...
Protein G purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent dilution.
||Use at an assay dependent dilution. Predicted molecular weight: 89 kDa.
Information by UniProt
- Centromere/kinetochore protein zw10 antibody
- Centromere/kinetochore protein zw10 homolog antibody
- HZW 10 antibody
Western blot - Anti-ZW10 antibody [3363C4a] (ab53676)
Staining of immunizing recombinant ZW10 fragment using anti-ZW10 antibody [3363C4a] (ab53676).Predicted band size:
89 kDaObserved band size:
38 kDa (why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
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