Overview

  • Product name

    Anti-ZWILCH antibody [EPR18035-28]
    See all ZWILCH primary antibodies
  • Description

    Rabbit monoclonal [EPR18035-28] to ZWILCH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ZWILCH aa 1-300. The exact sequence is proprietary.
    Database link: Q9H900

  • Positive control

    • WB: HEK-293, HeLa, PC-3, MCF7, C6, PC-12 and NIH/3T3 cell lysates; Mouse brain lysate. IP: HeLa whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18035-28
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IP 1/50.

Target

  • Function

    Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).
  • Sequence similarities

    Belongs to the ZWILCH family.
  • Cellular localization

    Chromosome, centromere, kinetochore.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ10036 antibody
    • FLJ16343 antibody
    • homolog of Drosophila Zwilch antibody
    • hZwilch antibody
    • KNTC1AP antibody
    • MGC111034 antibody
    • Protein zwilch homolog antibody
    • ZWILC_HUMAN antibody
    • zwilch antibody
    • zwilch kinetochore protein antibody
    • ZWILCH protein antibody
    • Zwilch, kinetochore associated, homolog antibody
    see all

Images

  • All lanes : Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution

    Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 15 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution + Mouse brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cells) cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast cells) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 15 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • ZWILCH was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab202898 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab202898 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. 

    Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab202898 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202898 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

ab202898 has not yet been referenced specifically in any publications.

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