RecombinantRabMAb

Recombinant Anti-ZWILCH antibody [EPR18035-28] (ab202898)

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Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
  • Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
  • Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
  • Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
  • Anti-ZWILCH antibody [EPR18035-28] (ab202898)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18035-28] to ZWILCH
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ZWILCH antibody [EPR18035-28]
    See all ZWILCH primary antibodies

  • Description

    Rabbit monoclonal [EPR18035-28] to ZWILCH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human ZWILCH aa 1-300. The exact sequence is proprietary.
    Database link: Q9H900

  • Positive control

    • WB: HEK-293, HeLa, PC-3, MCF7, C6, PC-12 and NIH/3T3 cell lysates; Mouse brain lysate. IP: HeLa whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18035-28

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IP 1/50.

Target

  • Function

    Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).

  • Sequence similarities

    Belongs to the ZWILCH family.

  • Cellular localization

    Chromosome, centromere, kinetochore.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ10036 antibody
      • FLJ16343 antibody
      • homolog of Drosophila Zwilch antibody
      • hZwilch antibody
      • KNTC1AP antibody
      • MGC111034 antibody
      • Protein zwilch homolog antibody
      • ZWILC_HUMAN antibody
      • zwilch antibody
      • zwilch kinetochore protein antibody
      • ZWILCH protein antibody
      • Zwilch, kinetochore associated, homolog antibody
      see all

    Images

    • Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
      Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
      All lanes : Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution

      Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate
      Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
      Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
      Lane 4 : MCF7 (Human breast adenocarcinoma cell line) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 67 kDa
      Observed band size: 67 kDa


      Exposure time: 15 seconds


      Blocking/dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
      Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
      Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution + Mouse brain lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 67 kDa
      Observed band size: 67 kDa


      Exposure time: 3 minutes


      Blocking/dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
      Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
      All lanes : Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution

      Lane 1 : C6 (Rat glial tumor cells) cell lysate
      Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
      Lane 3 : NIH/3T3 (Mouse embryo fibroblast cells) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 67 kDa
      Observed band size: 67 kDa


      Exposure time: 15 seconds


      Blocking/dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
      Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] (ab202898)

      ZWILCH was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab202898 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab202898 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. 

      Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab202898 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202898 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • Anti-ZWILCH antibody [EPR18035-28] (ab202898)
      Anti-ZWILCH antibody [EPR18035-28] (ab202898)

    References (0)

    Publishing research using ab202898? Please let us know so that we can cite the reference in this datasheet.

    ab202898 has not yet been referenced specifically in any publications.

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