Anti-ZWINT antibody (ab168501)
Key features and details
- Mouse polyclonal to ZWINT
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZWINT antibody
See all ZWINT primary antibodies -
Description
Mouse polyclonal to ZWINT -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human ZWINT aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. AAH20979.1
Database link: O95229 -
Positive control
- ZWINT transfected 293T cell lysate; Human liver lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. - Information by UniProt
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Database links
- Entrez Gene: 11130 Human
- Omim: 609177 Human
- SwissProt: O95229 Human
- Unigene: 591363 Human
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Alternative names
- Human ZW10 interacting protein 1 antibody
- HZwint 1 antibody
- HZwint1 antibody
see all
Images
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All lanes : Anti-ZWINT antibody (ab168501) at 1 µg/ml
Lane 1 : ZWINT transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 31 kDa -
Anti-ZWINT antibody (ab168501) at 1 µg/ml + Human liver lysate at 50 µg
Predicted band size: 31 kDa -
Immunofluorescent analysis of HeLa cells labeling ZWINT with ab168501 at 10 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab168501 has not yet been referenced specifically in any publications.