Key features and details
- Mouse polyclonal to ZWINT
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZWINT antibody
See all ZWINT primary antibodies
DescriptionMouse polyclonal to ZWINT
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
- ZWINT transfected 293T cell lysate; Human liver lysate; HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab168501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionPart of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase.
- Information by UniProt
- Human ZW10 interacting protein 1 antibody
- HZwint 1 antibody
- HZwint1 antibody
All lanes : Anti-ZWINT antibody (ab168501) at 1 µg/ml
Lane 1 : ZWINT transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 31 kDa
Anti-ZWINT antibody (ab168501) at 1 µg/ml + Human liver lysate at 50 µg
Predicted band size: 31 kDa
Immunofluorescent analysis of HeLa cells labeling ZWINT with ab168501 at 10 µg/ml.
ab168501 has not yet been referenced specifically in any publications.