Anti-ZWINT antibody (ab229692)
Key features and details
- Rabbit polyclonal to ZWINT
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ZWINT antibody
See all ZWINT primary antibodies -
Description
Rabbit polyclonal to ZWINT -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human ZWINT aa 1 to the C-terminus.
Database link: O95229 -
Positive control
- WB: HeLa whole cell lysate (ab150035); Mouse liver tissue lysate. IHC-P: Human ovarian cancer and testis tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Predicted molecular weight: 31 kDa. |
Target
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Function
Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. - Information by UniProt
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Database links
- Entrez Gene: 11130 Human
- Entrez Gene: 52696 Mouse
- Omim: 609177 Human
- SwissProt: O95229 Human
- SwissProt: Q9CQU5 Mouse
- Unigene: 591363 Human
- Unigene: 62876 Mouse
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Alternative names
- Human ZW10 interacting protein 1 antibody
- HZwint 1 antibody
- HZwint1 antibody
see all
Images
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All lanes : Anti-ZWINT antibody (ab229692) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Mouse liver tissue lysate
Secondary
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa -
Paraffin-embedded human ovarian cancer tissue stained for ZWINT using ab229692 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human testis tissue stained for ZWINT using ab229692 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229692 has not yet been referenced specifically in any publications.