Key features and details
- Rabbit polyclonal to ZWINT
- Suitable for: ICC/IF, WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZWINT antibody
See all ZWINT primary antibodies
DescriptionRabbit polyclonal to ZWINT
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to a region between residue 225 and the C terminus (residue 277) of human ZWINT (NP_008988.2).
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab71982 was affinity purified using an epitope specific to ZWINT immobilized on solid support.
Our Abpromise guarantee covers the use of ab71982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/250.
Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
|WB||1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPart of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase.
- Information by UniProt
- Human ZW10 interacting protein 1 antibody
- HZwint 1 antibody
- HZwint1 antibody
Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling ZWINT with ab71982 at at 1/200 (1µg/ml). A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.
All lanes : Anti-ZWINT antibody (ab71982) at 0.04 µg
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Detection of ZWINT by Western Blot of Immunprecipitate.
ab71982 at 1µg/ml staining ZWINT in HeLa whole cell lysate immunoprecipitated using ab71982 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab71982 has not yet been referenced specifically in any publications.