Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10905] to ZWINT
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-ZWINT antibody [EPR10905]
See all ZWINT primary antibodies
DescriptionRabbit monoclonal [EPR10905] to ZWINT
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ZWINT aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O95229
- MOLT4, A431, Raji and HeLa cell lysates. Immunoprecipitation pellet from Molt-4 cell lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 31 kDa.|
|IP||1/10 - 1/100.|
FunctionPart of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase.
- Information by UniProt
- Human ZW10 interacting protein 1 antibody
- HZwint 1 antibody
- HZwint1 antibody
All lanes : Anti-ZWINT antibody [EPR10905] (ab174266) at 1/1000 dilution
Lane 1 : MOLT4 cell lysates
Lane 2 : A431 cell lysates
Lane 3 : Raji cell lysates
Lane 4 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 31 kDa
Western blot analysis on immunoprecipitation pellet from Molt-4 cell lysate using ab174266 at a 1/10 dilution.
ab174266 has not yet been referenced specifically in any publications.