Product nameAnti-Zyxin antibody [EPR4302]
See all Zyxin primary antibodies
DescriptionRabbit monoclonal [EPR4302] to Zyxin
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Zyxin aa 300-400. The exact sequence is proprietary.
- WB: HeLa, MCF7 and C2C12 cell lysates IHC-P: Human kidney tissue ICC/IF: HeLa cells
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 82 kDa (predicted molecular weight: 61 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol.
|Flow Cyt||1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/500.|
RelevanceZyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
Cellular localizationCytoplasmic, cytoskeletal and Nuclear.
- ESP 2 antibody
- ESP2 antibody
- HED 2 antibody
Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labelling Zyxin with purified ab109316 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Overlay histogram showing HeLa cells stained with ab109316 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109316, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
All lanes : Anti-Zyxin antibody [EPR4302] (ab109316) at 1/10000 dilution
Lane 1 : HeLa lysate
Lane 2 : MCF7 lysate
Lane 3 : C2C12 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of Zyxin in paraffin embedded Human kidney tissue, using ab109316 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent staining of Zyxin in HeLa cells, using ab109316 at a 1/100 dilution.
This product has been referenced in:
- Han X et al. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules. Nat Commun 8:14639 (2017). Read more (PubMed: 28256511) »
- Qi L et al. Talin2-mediated traction force drives matrix degradation and cell invasion. J Cell Sci 129:3661-3674 (2016). Read more (PubMed: 27694340) »