Overview

  • Product name

    Anti-Zyxin antibody - N-terminal
    See all Zyxin primary antibodies
  • Description

    Rabbit polyclonal to Zyxin - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide within Human Zyxin (N terminal). The exact sequence is proprietary.
    Database link: Q15942

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab229757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    Zyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
  • Cellular localization

    Cytoplasmic, cytoskeletal and Nuclear.
  • Database links

  • Alternative names

    • ESP 2 antibody
    • ESP2 antibody
    • HED 2 antibody
    • HED2 antibody
    • ZYX antibody
    • ZYX protein antibody
    • Zyxin 2 antibody
    • Zyxin2 antibody
    see all

Images

  • All lanes : Anti-Zyxin antibody - N-terminal (ab229757) at 1/10000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : Zyxin Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 61 kDa

  • All lanes : Anti-Zyxin antibody - N-terminal (ab229757) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 61 kDa



    7.5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Zyxin (green) using ab229757 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229757 has not yet been referenced specifically in any publications.

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