Product nameAnti-Zyxin antibody [ZOL301]
See all Zyxin primary antibodies
DescriptionMouse monoclonal [ZOL301] to Zyxin
Tested applicationsSuitable for: WB, ELISA, ICC, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- Purchase matching WB positive control:Recombinant Human Zyxin protein
- Total cell extract of HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab50391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 61 kDa.|
|ELISA||Use at an assay dependent concentration.
Array: Use at an assay dependent dilution.
|ICC||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceZyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
Cellular localizationCytoplasmic, cytoskeletal and Nuclear.
- ESP 2 antibody
- ESP2 antibody
- HED 2 antibody
ab50391 staining rat neuronal progenitor cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X100 for 5 minutes prior to blocking in 10% serum at RT for 1 hour. The primary antibody was incubated with the sample for 1 hour at 4 µg/ml. An Alexa Fluor® 488 conjugated goat anti-mouse antibody diluted 1/1000 was used as the secondary.
This product has been referenced in:
- Han X et al. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules. Nat Commun 8:14639 (2017). Read more (PubMed: 28256511) »
- Lock FE et al. Differential regulation of adhesion complex turnover by ROCK1 and ROCK2. PLoS One 7:e31423 (2012). ICC/IF ; Human . Read more (PubMed: 22348083) »