Overview

  • Product name

    Anti-Zyxin antibody [ZOL301]
    See all Zyxin primary antibodies
  • Description

    Mouse monoclonal [ZOL301] to Zyxin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, ICC, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    PPQPREKVSSIDLEIDS

    conjugated to KLH by an N-terminal Cysteine residue linker, corresponding to amino acids 134-150 of Human Zyxin

  • Positive control

  • General notes

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab50391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
ELISA Use at an assay dependent concentration.

Array: Use at an assay dependent dilution.

ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Zyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
  • Cellular localization

    Cytoplasmic, cytoskeletal and Nuclear.
  • Database links

  • Alternative names

    • ESP 2 antibody
    • ESP2 antibody
    • HED 2 antibody
    • HED2 antibody
    • ZYX antibody
    • ZYX protein antibody
    • Zyxin 2 antibody
    • Zyxin2 antibody
    see all

Images

  • ab50391 staining rat neuronal progenitor cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X100 for 5 minutes prior to blocking in 10% serum at RT for 1 hour.  The primary antibody was incubated with the sample for 1 hour at 4 µg/ml.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody diluted 1/1000 was used as the secondary.

    This image was generated using the ascites version of the product.

    See Abreview

References

This product has been referenced in:

  • Zhao R  et al. Endothelial cells promote metastasis of prostate cancer by enhancing autophagy. J Exp Clin Cancer Res 37:221 (2018). Read more (PubMed: 30200999) »
  • Han X  et al. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules. Nat Commun 8:14639 (2017). Read more (PubMed: 28256511) »
See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (neuronal progenitor cell)
Specification
neuronal progenitor cell
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X100 for 5 minutes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Dr. Anne Chiaramello

Verified customer

Submitted Apr 18 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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