Key features and details
- Rabbit polyclonal to Zyxin (phospho S142)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Zyxin (phospho S142) antibody
See all Zyxin primary antibodies
DescriptionRabbit polyclonal to Zyxin (phospho S142)
Specificityab78910 detects endogenous levels of Zyxin only when phosphorylated at serine 142.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Synthetic phosphopeptide derived from human Zyxin around the phosphorylation site of serine 142 (K-V-SP-S-I)
- Extracts from Jurkat cells treated with paclitaxel (1uM, 24hours).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab78910 was purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab78910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 61 kDa.|
RelevanceZyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
Cellular localizationCytoplasmic, cytoskeletal and Nuclear.
- ESP 2 antibody
- ESP2 antibody
- HED 2 antibody
All lanes : Anti-Zyxin (phospho S142) antibody (ab78910) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells, treated
with paclitaxel (1uM, 24hours)
Lane 2 : Extracts from Jurkat cells, treated
with paclitaxel (1uM, 24hours) with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
ab78910 has been referenced in 1 publication.
- Bernusso VA et al. Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells. Biochim Biophys Acta 1853:388-95 (2015). PubMed: 25450971