Key features and details
- Rabbit polyclonal to ZZEF1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZZEF1 antibody
See all ZZEF1 primary antibodies
DescriptionRabbit polyclonal to ZZEF1
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- Human testis tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityExpressed at low levels in cerebellum.
Sequence similaritiesContains 1 DOC domain.
Contains 1 EF-hand domain.
Contains 2 ZZ-type zinc fingers.
- Information by UniProt
- FLJ10821 antibody
- KIAA0399 antibody
- Zinc finger ZZ-type and EF-hand domain-containing protein 1 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab122194 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122194 at 1/400 dilution staining ZZEF1 in Paraffin-embedded Human Testis tissue by Immunohistochemistry.
ab122194 has not yet been referenced specifically in any publications.