Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityExpressed at low levels in cerebellum.
Sequence similaritiesContains 1 DOC domain.
Contains 1 EF-hand domain.
Contains 2 ZZ-type zinc fingers.
- Information by UniProt
- FLJ10821 antibody
- KIAA0399 antibody
- Zinc finger ZZ-type and EF-hand domain-containing protein 1 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab122194 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122194 at 1/400 dilution staining ZZEF1 in Paraffin-embedded Human Testis tissue by Immunohistochemistry.
ab122194 has not yet been referenced specifically in any publications.