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Rabbit polyclonal to ZZEF1
Predicted to work with:
Synthetic peptide within Human ZZEF1 aa 525-575. The exact sequence is proprietary. NP_055928.3
EDKSGPENLL VEPWTRDGFL TETGKTRAST IFSTG
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
ab176594 not performed satisfactorily when used for WB of ZZEF1 in crude preparations (e.g. whole cell lysate), however can be used for WB of enriched (e.g. immunoprecipitated) sources of ZZEF1.
Is unsuitable for WB.
Expressed at low levels in cerebellum.
Contains 1 DOC domain.
Contains 1 EF-hand domain. Contains 2 ZZ-type zinc fingers.
Information by UniProt
Zinc finger ZZ-type and EF-hand domain-containing protein 1 antibody
Immunoprecipitation - Anti-ZZEF1 antibody (ab176594)
Detection of ZZEF1 by Western Blot of Immunprecipitate.
ab176594 at 0.4µg/ml labeling ZZEF1 in 293T whole cell lysate immunoprecipitated using ab176594 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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